FREEDOM OF EXPRESSION AS A PREMISE FOR THE DIVERSITY OF CULTURAL EXPRESSIONS


Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

CRISPR/Cas9 is a powerful tool for genome editing.We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose.In our experiments, we targeted two different genes, screened here for mutations hacklinkci.com and

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2D Diagonal Redox SDS-PAGE of Proteins

2D diagonal redox koip share price SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012).Both dimensions consist of a conventional SDS-PAGE, except that the sample buffer for the first dimension lacks a reducing agent.Intermolecu

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